During 2018 in the implementation of the A2 action of the LIFE + RIPISILVANATURA project, work-meetings with the participation of more than 30 experts and staff involved in the environmental management have been developed to elaborate priority lists of aquatic and riparian Invasive Alien Species (IAS) in the Segura River Basin. In the present work we summarize the results of this participative evaluation action that has been innovative because it comes to improve communication and discussion channels at a regional level. In addition, this action has combined the interest of the Environmental Administration to update priorities in the management and control of IAS with the collaborative work of experts. Through the application of species selection criteria and the prioritization by experts, a priority list of IAS for the management in the Segura River Basin composed of a total of 47 taxa (27 fauna and 20 flora) has been obtained. In addition, the first alert list of IAS with invasive potential (115 taxa) has also been developed, which should be a basic tool when making decisions in the strategy against new invasions. This work has received the grant through the LIFE program (LIFE13 BIO / ES / 001407).